Omics technologies : tools for food science

OMICs Technologies: Tools for Food Science
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Published Date: 4th June Page Count: View all volumes in this series: Comprehensive Analytical Chemistry.

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Institutional Subscription. Free Shipping Free global shipping No minimum order. Describes the latest applications of omics technologies Provides an excellent reference for applications of advanced omics techniques Includes advanced tools and methodologies for dealing with the data generated. Powered by.

About Preventomics - Personalised nutrition plans accessible for everyone

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The old password does not match our records. Changing behaviour programme. Thanks in advance for your time. You must be an instrument owner to approve or reject users. A new password has been sent to your email. View All Capillary Electrophoresis. Track Food Science and Chemistry Food science is the applied science devoted to the study of food.

Alejandro has given more than invited lectures in different national and international meetings in Europe, Asia, Africa, America and Oceania. He has defined for the first time in a SCI journal the new discipline of Foodomics. She has published over 50 SCI papers and 10 book chapters. Her h index is 20 and her works have received over citations August We are always looking for ways to improve customer experience on Elsevier.

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View on ScienceDirect. Hardcover ISBN: Imprint: Elsevier. Published Date: 4th June However, if used in a complementary way these techniques are promising for providing an integrated picture of the relationship between composition and activity of soil microflora.

Review ARTICLE

OMICS Technologies: Tools for Food Science explores how these tools reveal the fundamental pathways and biochemical processes that drive. Since the completion of the Human Genome Project, food and nutrition sciences have undergone a fundamental molecular transformation. New discoveries in.

The rhizosphere is a nutrient rich environment, where numerous interactions between plant and microorganisms occur, ranging from mutualism to parasitism. The enrichment of specific microbial populations in the rhizosphere is dependent on the capability of these micro-organisms to utilize root exudates, to effectively colonize the root surface and to interact or compete with other micro-organisms.

Analysis of the rhizospheric communities incorporating both established techniques, and recently developed "omic technologies" can now facilitate investigations into the molecular basis underpinning the establishment of plant-microbial interactomes in the rhizosphere. Therefore, the aim of this chapter is to present an overview of bacterial functions enriched in the rhizosphere of different plant species using data obtained from several functional genomics analyses.

Metagenomics has been defined as the study of the collective genomes of the microbiota in given habitat. Soil offers a huge microbial diversity and the use of metagenomics approaches will allow a deeper understanding of soil microbial diversity and function. The two areas, phylogenetically-based diversity and functional gene based function, are complementary and may be used side-by-side in order to allow a better understanding of the living soil. Moreover, genes for relevant functions can be cloned into suitable vectors, after which they can be studied and possibly explored for biotechnological purposes.

Thus, opportunities for novel product discovery via metagenomics are rapidly rising. However, there are caveats in what metagenomics techniques can tell us about the soil environment and its functioning, and also in the chances of successful exploration of soil. In this chapter, we review the developments in the metagenomics-based exploitation and exploration of soil and examine how soil metagenomics can enhance our vision about natural functioning and exploration for biotechnological novelty.

Table of Contents

One major issue, the need for advanced bioinformatics tools, is stressed. We conclude that the rich microbiota of soil offers an astonishing big playground for metagenomics, but that methodological and conceptual problems still hamper its full exploitation.

Many of the ecosystem services are soil associated with microbes playing a predominant role. Nevertheless, our current knowledge of microbial contribution to ecosystem processes is still limited, partly because in the past centuries research was mostly based on culture-dependent methods, being oblivious of the vast un-cultivable microbial majority as proven during the last decades. Current molecular biology advances provide us with the ability to screen for microbial identities or functions by targeting marker genes in nucleic acid extracts of environmental samples, therefore partly bypassing previous methodological limitations.

Topics addressed here aim at providing an overview of methodologies and concepts related to marker gene screening from environmental samples. Such are the description of marker gene categories, examples of their use in soil environments and the description of marker gene screening state-of-the-art methodologies and specifications. Finally we will exemplify the use of late methodologies for the case of the bacterial small ribosomal subunit screening in soil environments.

Metatranscriptomics is defined as the analysis of microbial community gene expression in a particular environment, as opposed to metagenomics which is the study of the genomic content of entire microbial communities. Massively parallel sequencing of RNA is the key component of metatranscriptomics.

The analysis of enriched mRNA has the potential to discover novel genes and to uncover functional adaptations of microbial communities to local environmental conditions. Alternatively, total RNA may be used for analysis. This approach provides insight into the taxonomic composition of microbial communities. The subject of this review is soil metatranscriptomics. We discuss the experimental and bioinformatic workflow that can be applied to the metatranscriptomic analysis of soil microbial communities.

Since research in the field of soil metatranscriptomics is still in its infancy, we also review the recent advances in marine metatranscriptomics. Proteomics is a potent post genomic approach with the potential to interrogate natural complex systems such as soils.