Rapid Immunotests for Clinical, Food and Environmental Applications
Free download. Book file PDF easily for everyone and every device. You can download and read online Rapid Immunotests for Clinical, Food and Environmental Applications file PDF Book only if you are registered here. And also you can download or read online all Book PDF file that related with Rapid Immunotests for Clinical, Food and Environmental Applications book. Happy reading Rapid Immunotests for Clinical, Food and Environmental Applications Bookeveryone. Download file Free Book PDF Rapid Immunotests for Clinical, Food and Environmental Applications at Complete PDF Library. This Book have some digital formats such us :paperbook, ebook, kindle, epub, fb2 and another formats. Here is The CompletePDF Book Library. It's free to register here to get Book file PDF Rapid Immunotests for Clinical, Food and Environmental Applications Pocket Guide.
Contents:
Selective capture and rapid identification of E. RSC Advances , 7 48 , Food Analytical Methods , 9 4 , A novel biosensor for Escherichia coli OH7 based on fluorescein-releasable biolabels.
Purchase Rapid Immunotests for Clinical, Food and Environmental Applications, Volume 72 - 1st Edition. Print Book & E-Book. Rapid Immunotests for Clinical, Food and Environmental Applications, Volume 72 (Comprehensive Analytical Chemistry): Medicine & Health.
Biosensors and Bioelectronics , 78 , Nanosensors for the Detection of Pathogenic Bacteria. Rapid Multiplex Immunotests. Tsougeni, G.
Loop-mediated isothermal amplification of DNA. Products of this store will be shipped directly from the UK to your country. Be the first to write a review. This single strand with primer can enter the multipriming amplification cycle; thus there is no dead-end product and amplification will be sustained until the reagents become exhausted. A self-powered, one-step chip for rapid, quantitative and multiplexed detection of proteins from pinpricks of whole blood.
Papadakis, M. Gianneli, A.
Join Kobo & start eReading today
Grammoustianou, V. Constantoudis, B. Dupuy, P. Petrou, S. Kakabakos, A. Tserepi, E. Gizeli, E. Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis. Lab on a Chip , 16 1 , SERS aptasensor detection of Salmonella typhimurium using a magnetic gold nanoparticle and gold nanoparticle based sandwich structure. Analytical Methods , 8 45 , Functionalized polyurethane applied for foodborne pathogen detection. Journal of Food Measurement and Characterization , 9 3 , Erdogan Ceylan.
Rapid detection of Listeria monocytogenes in milk using confocal micro-Raman spectroscopy and chemometric analysis. International Journal of Food Microbiology , , Gupta, Sanjeev K. Bhure, Premanshu Dandapat, Rajesh K. Agarwal, Vijendra P. Anaerobe , 33 , Gupta, P. Gulati, N. Bhagat, M. Dhar, J. Detection of Yersinia enterocolitica in food: an overview.
Sensors , 15 3 , A sensitive lateral flow biosensor for Escherichia coli OH7 detection based on aptamer mediated strand displacement amplification. Analytica Chimica Acta , , Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles.
Rapid Immunotests for Clinical, Food and Environmental Applications: Volume 72
The Analyst , 4 , Advances in Natural Sciences: Nanoscience and Nanotechnology , 5 4 , Facile synthesis of multifunctional multi-walled carbon nanotube for pathogen Vibrio alginolyticus detection in fishery and environmental samples. Talanta , , Michael Seidel, Reinhard Niessner. Chemiluminescence microarrays in analytical chemistry: a critical review. Analytical and Bioanalytical Chemistry , 23 , N-methylimidazolium functionalized magnetic particles as adsorbents for rapid and efficient capture of bacteria.
Microchimica Acta , , Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing. Biosensors and Bioelectronics , 58 , Gupta, Seema Sood, B. Biosensors for pathogen detection: A smart approach towards clinical diagnosis.
Bestselling Series
Sensors and Actuators B: Chemical , , A universal fluorescent aptasensor based on AccuBlue dye for the detection of pathogenic bacteria. Fluorescent aptasensor for the determination of Salmonella typhimurium based on a graphene oxide platform. Elazar Fallik. Microbial Quality and Safety of Fresh Produce.
- Rapid Immunotests for Clinical, Food and Environmental Applications, Volume 72 - 1st Edition.
- Combinatorial Group Testing and Its Applications.
- Aptamers as a new alternative to antibodies - labor&more.
Electrochemical immunosensors, genosensors and phagosensors for Salmonella detection. Methods , 6 22 , Paniel, J. Baudart, A. Hayat, L. Aptasensor and genosensor methods for detection of microbes in real world samples. Methods , 64 3 , Integrated sorting, concentration and real time PCR based detection system for sensitive detection of microorganisms. Scientific Reports , 3 1 DOI: Analytical Letters , , Protein G-based surface chemistry when used together with NeutrAvidin-based surface chemistry enables the separation of capture and detection chemistries that can potentially reduce the nonspecific binding and enhance detection outcomes Their immunosensing device enables the development of POC on-chip technologies to monitor viral load and guide antiretroviral treatment ART in resource-constrained settings [ ].
The platform features a high degree of modularity and integration. Modularity allows the adaption of common and established assay types of various formats. Integration lets the system move from the laboratory to the point-of-care settings with multiplexing capability.
By making use of the microarray format, the lab-on-chip system also addresses new trends in biomedicine. The low-cost device has reagents reservoirs, microfluidic actuators, and various sensors integrated within the cartridge. In combination with fully automated instrumentation read-out and processing unit , a diagnostic assay can be performed in about 15 minutes.
This is possible with user-friendly interfacing read-out unit, data acquisition, and data analysis units together. The assays for nucleic acids detection of different pathogens and protein markers such as CRP and PSA have been done using an electrochemical sensor based on redox cycling or an optical sensor based on total internal reflectance fluorescence TIRF. Furthermore, integration of sample preparation and polymerase chain reaction PCR on-chip has been done. The instrument is capable of providing heating-and-cooling cycles necessary for DNA amplification [ ].
Electrochemical and optical technologies are the clear leaders in detection technologies in the market as they do not use complicated instrumentation for detection. Therefore point-of-care detection should be able to detect signal without using any complicated instrumentation or if the results can be visualized.
Optical detection is the simplest and most popular method used in immunoassay applications. Most immunosensors are based on optical detection and commonly use a label e. Optical immunosensors combined with microfluidic chips have recently been proposed as an attractive immunosensing platform, and many reviews have explored their potential applications in clinical diagnostics, particularly because there is a growing need to simultaneously screen multiple proteins in a single sample.
The optical detection method, which can be easily implemented in microfluidic systems, is a prime candidate for this multiplexed analysis. Optical detection methods can be divided into five main categories on the basis of detection signal: fluorescence, luminescence, absorbance colorimetry , surface plasmon resonance SPR , and surface-enhanced Raman scattering SERS ; each technique has inherent advantages and disadvantages. In this section, we focus on the recent multiplexing applications in microfluidic immunosensors using optical detection techniques.
There are several optical detection methods used for POC applications such as fluorescence with variants such as Forster resonance energy transfer FRET and upconverting phosphor technology, luminescence, absorbance colorimetry , surface-plasmon resonance SPR , and various categories of light scattering: Rayleigh particles much smaller than wavelength , Mie particles comparable to wavelength, shape dependent , geometric particles larger than wavelength , resonant wavelength overlaps an electronic transition of the particle , and Raman vibrational quanta added to or subtracted from the excitation wavelength [ ].
The most commonly used technique is absorbance as it is commonly used in LFAs based on gold or polymer nano- particles. However fluorescence is used for the broadest range of different types of POC assays [ ] for reasons of sensitivity and, more recently, the ready availability of a range of different colors of efficient fluorophores, including quantum dots, quantum-dot barcodes, and fluorescent nanoparticles, providing improved limits of detection. Fluorescence in some cases can detect single particle LODs enabling multitarget multiplexing [ ].
The supercritical angle fluorescence SAF which is being used recently detects fluorescence emitted in close proximity to a fluorophore-supporting optically transparent chip surface. This method provides substantial enhancement of fluorescence collection efficiency while rejecting background from unbound fluors or impurities, as it confines the fluorescence detection volume to material within about one wavelength of the chip surface [ ].
Nanoparticles including quantum dots are finding increasing application. Nanoparticle labels conjugated with biomolecules have been used in a variety of different assay application. Nanoparticles offer adjustable and expandable reactive surface area compared to the more traditional solid phase forms utilized in bioaffinity assays due to the high surface-to-volume ratio. Signal enhancement by conjugating nanoparticles with fluorescent, luminescent, and other measurable properties has enhanced detection limit by several folds.
The potential to multiplex along with the ability to increase sensitivity and specificity without using enzymes has increased the use of nanoparticles in immunoassays for early detection. It has been shown that using time resolved fluorescence of lanthanides like europium nanoparticles with long stokes shift can reduce background and increase detection limit to as low as 0.
The determination of cancer biomarkers in serum and saliva using quantum dot bioconjugate labels are used. Aptamers were tethered to gold nanoparticles as part of an LFA-like dry-reagent assay strip to detect thrombin [ ]. Thermal-lens microscopy TLM , an alternative to fluorescence detection, also uses dye labeling for detection. Electrochemical immunoassays are the most commonly used analytical techniques for the quantitative detection of biomolecules, followed by optical methods.
Electrochemical immunosensors are not only sufficient to meet the demands for sensitive, rapid, and selective determination of analytes but can also be incorporated into robust, portable, and miniaturized devices.
Log in to Wiley Online Library
Specifically, the integration of electrochemical detection with microfluidic chips offers an attractive immunoassay platform with significant advantages derived from the combination of electrochemical analysis and a microfluidic system [ ]. In broad terms, electrochemical immunosensors function by detecting an electrical signal that arises from specific immunoreactions that occur at the surface of an electrode. According to the type of electrical detection signal, electrochemical techniques are classified into three basic categories: voltammetry current , potentiometry potential shift , and impedimetry resistance [ ].
It has been shown that ultrasensitive capacitive immunosensor is capable of detecting subattogram per milliliter concentrations of p24 antigen [ ]. Piezoelectric devices convert a physical or mechanical change into electrical energy and vice versa.
The commonest piezoelectric sensor is the quartz crystal microbalance QCM , which exploits the change in the resonance of quartz crystals upon changes in their mass, allowing binding of antigen to antibody when one of these is immobilised on the crystal surface to be measured electrically [ ].
- The Artists Reality
- Race Rebels: Culture, Politics, and the Black Working Class
- Lévy Matters I: Recent Progress in Theory and Applications: Foundations, Trees and Numerical Issues in Finance
- Remolding and Resistance Among Writers of the Chinese Prison Camp: Disciplined and published (Routledge Studies in Asias Transformations)
- Scenes from an Unholy War (Vampire Hunter D Novels, Book 20)